All in One Grow Bag Instructions

As passionate advocates for mushroom cultivation, we prioritize your safety above all else. Before handling mushrooms or mushroom products, take a moment to review our safety checklist:

  • Allergy Awareness: Be mindful of potential allergic reactions to mushrooms. If you or anyone in your household has a known mushroom allergy, exercise caution or avoid handling mushrooms altogether.

  • Contamination Prevention: Keep your workspace clean and sanitized to prevent contamination. Regularly wash your hands and disinfect surfaces to minimize the risk of harmful bacteria or mold growth. 

  • Contamination Protocol: In the event that a product is contaminated, take immediate action. Remove the contaminated item from your workspace and dispose of it properly. Thoroughly clean and sanitize any affected surfaces to prevent further contamination.
  • Needle Safety: Exercise extreme caution when using needles or other sharp tools in mushroom cultivation. Always handle them with care, and store them securely to prevent accidental injuries.

Remember, your safety is paramount to us. By following these simple guidelines, you can enjoy the rewarding experience of mushroom cultivation with peace of mind. Happy growing!

Items in the Box:
1. All in one Grow Bag with substrate, grain and self healing injection port
2. 10ml Liquid Culture 
3. Syringe needle
4. Alcohol wipe
5. Misting bottle

Items Required:
1. Mask
2. Disposable or reusable gloves (Alternatively, thoroughly wash hands with warm water and soap)
3. Lighter or Match
4. Sharp knife

The kit has a shelf life of 1-2 weeks. If not inoculating immediately, store the bag upright at room temperature to facilitate air exchange.
For storage of the syringe, place it in an area of the fridge, away from direct airflow and other food.
Note that Pink Oyster and Yellow Oyster cultures cannot be refrigerated.

Our bags are intended for immediate use, as they arrive without pre-colonization. It is advisable to refrain from refrigeration unless the bags have reached full colonization. Once fully colonized, they can be safely stored in the refrigerator until they are prepared for fruiting. Further details on the colonization process will be provided in subsequent sections.

  1. Begin by unpacking the mushroom kit, removing the protective wrap, and gently unfolding the top flap to allow fresh air through the 0.3-micron filter patch.
  2. Prior to inoculation, inspect the bag for any signs of contamination, leaks, or holes.
  3. Sterilize the injection port with the provided  alcohol wipe to minimize the risk of contamination.
  4. Prepare your liquid culture solution for inoculation, shaking the syringe vigorously to break up the mycelium.
  5. Sterilize the syringe by holding it in a flame until red hot, then allow it to cool for 15 seconds. Avoid inserting a hot needle into the bag.
  6. Insert the cooled needle into the injection port and slowly release 10ml of the culture into the bag in multiple directions to ensure even even spread through the grain.
  7. If the bag doesn’t have an injection port, simply insert the cooled needle into the bag aiming at different directions in the grain at the bottom of the bag. Then quickly tape over your injection point with micropore, duct or any household tape. This will protect the bag from contamination.
  8. Remove the needle and gently rotate the bag in all directions to distribute the mycelium evenly into the nutrient medium. Do not shake vigorously or mix the substrate with grains at this stage.
  9. Place the inoculated kit in a warm, dark location with an incubation temperature of approximately 18-24 degrees Celsius for incubation.
  1. After 3-5 days, you should see white mycelium growing on the grain at the bottom of the kit, showing it’s getting ready.
  2. Gently squeeze the bottom of the bag to spread the colonized grains into the uncolonized grains. This will speed up the colonization process.
  3. Let the mycelium (a network of fungal threads) grow on all the grain before moving on.
  4. Once the mycelium has spread, mix the grain and compost in the kit well. Be patient and wait for everything to be fully covered.
  5. It’s best to fruit the kit when 80% or more of the substrate is covered with mycelium. Waiting for 100% is even better.

The three essential factors for successful fruiting are temperature, oxygen level, and humidity. Maintaining these at the right levels will encourage the substrate to fruit.
Once the mushroom kit is fully colonized, you have different options for fruiting.
1. You can fruit the kit inside the bag or transfer it to a terrarium, plastic tote, fruiting chamber, or monotub.
2. If you choose to fruit in the bag, place it in a humid room like the Laundry with natural light then cut a X on one side of the bag (top or side) to open and let in fresh air. Cut the bag below the heat seal on top for Enoki, Giant King and Golden Enoki. 
3. Soak the substrate in cold water for 4-6 hours with the cut X facing the water. This will allow the substrate to absorb the water it needs.
4. Drain the excess water out and place the bag in an well lit area with free air movement.
5. Keep the substrate moist by misting the cut X with clean water twice a day. Ideally use cooled boiled water.
6. Ideal temperature depends on the mushroom species but is usually around 21-24 degrees Celsius.
7. It usually takes about 10-16 days for mushrooms to start growing when it’ll start the ‘pinning’ phase when baby mushrooms start to grow.
8. You don’t need to mist when the pinning phase starts.
9. As the mushrooms grow, open the bag more to give them space.
10. Depending on the species, usually with in a week after pinning has started, the mushrooms will be ready for harvest.

1. Once the first harvest is complete, repeat from step 3 to step 10 from Stage 3 (above). 
2. Start by soaking the substrate in cold water for 4-6 hours with the cut X facing the water.

Once second harvest is complete, third and fourth harvests are possible but chances of contamination increases with every harvest.